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MRM/PRM靶向蛋白質(zhì)組


定量蛋白質(zhì)組的結(jié)果(無論是標(biāo)記定量還是非標(biāo)記定量)往往需要通過其他的實(shí)驗(yàn)給予證實(shí)。傳統(tǒng)的蛋白質(zhì)組數(shù)據(jù)的驗(yàn)證通過基于抗體的分析方法(例如,Western blot或ELISA)。而多反應(yīng)監(jiān)測技術(shù)(multiple reaction monitoring,MRM)由于其獨(dú)特優(yōu)勢,成為了驗(yàn)證定量蛋白質(zhì)組數(shù)據(jù)的主要分析手段。

 

MRM/PRM技術(shù)集中表現(xiàn)在三大優(yōu)勢:

1. 基于質(zhì)譜信號(hào)響應(yīng)的定量信息遠(yuǎn)比基于圖像信號(hào)的定量分析更為準(zhǔn)確;

2. 一次MRM分析可完成多個(gè)靶標(biāo)蛋白質(zhì)的定量測定;

3. 實(shí)驗(yàn)費(fèi)用經(jīng)濟(jì),節(jié)省時(shí)間。 

相關(guān)文獻(xiàn):

Wang Y et al. Discovery of potential colorectal cancer serum biomarkers through quantitative proteomics on the colonic tissue interstitial fluids from the AOM–DSS mouse model[J]. Journal of Proteomics, 2016, 132:31-40.

http://www.sciencedirect.com/science/article/pii/S1874391915301834

 

Zi J, Zhang J, Wang Q, et al. Stress responsive proteins are actively regulated during rice (Oryza sativa) embryogenesis as indicated by quantitative proteomics analysis[J]. PloS one, 2013, 8(9): e74229.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074229